LILRE oligoprecipitation of nuclear extracts isolated from AML cells and monocytes.

Lanes 1, 3, 5, 7, 9, and 11 represent control precipitations using streptavidin sepharose beads with no bound LILRE oligonucleotides. Lanes 2, 4, 6, 8, 10, and 12 show precipitations using LILRE-coupled streptavidin sepharose beads. (A) Nuclear extracts isolated from unstimulated (lanes 1, 2, 7, and 8), IL-6–stimulated (lanes 3, 4, 9, and 10), and IFN-γ–stimulated (lanes 5, 6, 11, and 12) AML cells followed by immunoblotting using pY-STAT1 and pY-STAT3 antibodies. Each precipitation was performed using 50 μg nuclear protein. (B) Nuclear proteins isolated from unstimulated (lanes 1, 2, 7, and 8), IL-6–stimulated (lanes 3, 4, 9, and 10), and IFN-γ–stimulated (lanes 5, 6, 11, and 12) monocytes. Each precipitation was performed using 100 μg nuclear protein. Figures are representative of 3 independent experiments (n = 3). MW indicates molecular weight.