Fig. 3.
In vivo and in vitro phosphorylation of FANCA coimmunoprecipitated with FANCG.
(A) Cell lysates from 32P-labeled EUFA316, EUFA316/wt-FANCG, EUFA143, and EUFA143/wt-FANCG cells were subjected to immunoprecipitation (IP) with anti-FANCG antibody. An arrow indicates phosphorylated FANCA. The lower panels show Western blots of FANCA and FANCG. (B) Cell lysates from HSC72, HSC72/wt-FANCA, GM6914, and GM6914/wt-FANCA were subjected to immunoprecipitation (IP) with preimmune serum (Pre) or anti-FANCG antibody; then in vitro phosphorylation of the immunoprecipitates was analyzed. An arrow indicates phosphorylated FANCA. The lower panels show Western blots of FANCA and FANCG.
