American Society of Hematology

Fig. 6.

$Fig. 6. Cytoplasmic localization of FANCA phosphorylation. / (A) Cytoplasmic localization of phosphorylated FANCA and FANCA-PK activities. HSC72/wt-FANCA cells (approximately 3 × 107cells) were metabolically labeled by 32P and fractionated to cytoplasmic and nuclear fractions; then each fraction was subjected to immunoprecipitation with anti-FANCA antibody. In vitro FANCA phosphorylation was examined using each fraction from HSC72/wt-FANCA cells (approximately 3 × 107 cells). The middle panel shows Western blots of FANCA. In parallel experiments, each fraction from the cells (approximately 5 × 105 cells) was subjected to Western blotting with antibodies against c-Abl and β-tubulin, nuclear and cytoplasmic markers, respectively (lower panels). (B) Structure of FANCA(delNLS). (C) In vivo phosphorylation of FANCA(delNLS) in GM6914. Western blots of FANCA are shown at the bottom. (D) Phosphopeptide mapping of wt-FANCA and FANCA(delNLS) phosphorylated in vitro.$

Cytoplasmic localization of FANCA phosphorylation.

(A) Cytoplasmic localization of phosphorylated FANCA and FANCA-PK activities. HSC72/wt-FANCA cells (approximately 3 × 10⁷cells) were metabolically labeled by ³²P and fractionated to cytoplasmic and nuclear fractions; then each fraction was subjected to immunoprecipitation with anti-FANCA antibody. In vitro FANCA phosphorylation was examined using each fraction from HSC72/wt-FANCA cells (approximately 3 × 10⁷ cells). The middle panel shows Western blots of FANCA. In parallel experiments, each fraction from the cells (approximately 5 × 10⁵ cells) was subjected to Western blotting with antibodies against c-Abl and β-tubulin, nuclear and cytoplasmic markers, respectively (lower panels). (B) Structure of FANCA(delNLS). (C) In vivo phosphorylation of FANCA(delNLS) in GM6914. Western blots of FANCA are shown at the bottom. (D) Phosphopeptide mapping of wt-FANCA and FANCA(delNLS) phosphorylated in vitro.

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