Effect of antisense oligonucleotides directed against PKCζ.

KG1a cells were pre-incubated with 10 μM antisense (AS) PKCζ or sense (control) oligonucleotides for 48 hours and then were treated or not treated with CH11 (2 μg/mL). (A) PKCζ expression analyzed by Western blot. (B) Fas-induced cell cytotoxicity was evaluated by Trypan blue exclusion assay. Results are the mean ± SD of 3 independent experiments. *P < .05. (C) Caspase-8 activity was evaluated at 4 hours after CH11 treatment, as described in “Materials and methods.” Results are expressed as percentage increase in CH11 against IgM-treated cells and are the mean ± SD of 3 independent experiments. *P < .05. (D, E) Morphology of CH11-treated cells was analyzed by fluorescence microscopy after DAPI staining. (D) Sense oligonucleotide-treated KG1a cells. (E) Antisense oligonucleotide-treated KG1a cells.