Par-4 and PKCζ expression and PKCζ activity in KG1a/G8 cells.

(A) Par-4 expression was analyzed by Western blot in KG1a, KG1a/G8, and Jurkat cells. (B) KG1a or KG1a/G8 cellular extracts were immunoprecipitated with anti-PKCζ monoclonal antibody. PKCζ activity was determined, as described in “Materials and methods,” using histone H1 as substrate. In parallel, an aliquot of each sample was analyzed by Western blot using anti-PKCζ monoclonal antibody to quantify immunoprecipitated proteins. (C) PKCζ expression was analyzed by Western blot in KG1a and KG1a/G8 cells.