Fas sensitivity of KG1a/G8 cells.

(A) Caspase-8 activity was evaluated as described in “Materials and methods.” Results are expressed as percentage increase in CH11 against IgM-treated cells and are the mean ± SD of 3 independent experiments. *P < .05. (B) Caspase-8 cleavage in KG1a/G8 cells treated by CH11 or IgM (2 μg/mL for 4 hours). (C) After treatment by 2 μg/mL CH11 (■) or IgM (□), KG1a/G8 cell viability was evaluated by Trypan blue exclusion assay. Results are the mean ± SD of 3 independent experiments. *P < .05. (D, E) Morphology of CH11-treated cells was analyzed by fluorescence microscopy after DAPI staining. (D) KG1a cells. (E) KG1a/G8 cells.