Expression of normal KB3 and KB3 Walton in transfected MDCK cells.

MDCK cells stably transfected with KB3 (A,B) or KB3 Walton (C,D) were treated for immunofluorescence using the antiband 3 Bric170 (A,C) or the antiband 3 Bric155 (B,D) and fluorescein isothiocyanate–conjugated rabbit antimouse secondary antibody as described in “Patients, materials, and methods.” The cells were imaged using confocal microscopy. Representative fields of view are shown. Bright field microscopy showed that all the cells in fields A through C were reactive with the antibody and fields C and D contained a similar number of cells. Bar = 10 μM.