Sequence alignment and analysis of the human and murine STAT3 genes.

Exon 22 to exon 23 in the human and murine STAT3 genes was aligned. Sequence analysis identified the possible 5′ splice donor site and possible adenosine BPSs, PPTs, and 3′ ASs for generating STAT3α and STAT3β mRNA. The critical nucleotides in each element are in boldface type. In addition, cryptic δ and ε 3′ splice ASs were detected. The δ 3′ AS presumably uses the same BPS and PPT as α. For ε, the possible BPS and PPT are indicated. An asterisk indicates nucleotide homology. The regions of intron 22 deleted in the ΔA, ΔB, ΔC, and ΔD constructs are indicated. The GenBank accession numbers for the murine and human sequences are AF332507 and AF332508, respectively.