Effect of exon mutations on exon 16 splicing during MEL cell induction.

In constructs E7 and GE, exon 16 was substituted by the constitutive 4.1R exon 7 or β-globin exon 2, respectively. In construct Ch1, the putative exon splicing silencer from exon 16 was inserted in the globin exon. In all these constructs, the flanking intronic sequences are identical to the WT intronic context. See also the legend to Figure 4.