Effect of downstream intron mutations on exon 16 splicing in induced MEL cells.

In constructs DI-Nd2, DI-Pv, and DI-Nr, the 3 UGCAUG motifs were targeted. *Construct DI-Nd2 contains the mutated UGCAUG in duplicate. Note that the effect on exon inclusion occurs more specifically in induced cells for most of the mutants. For constructs DI-Bb and DI-Bc, exon selection is clearly inhibited in both uninduced and induced cells. See also the legend to Figure 4.