FACS analysis of blood leukocytes, peritoneal cavity, and pleural cavity cells.

(A) Typical FACS analyses of monocytes, granulocytes, and lymphocytes by forward and side light scatter. Separate regions encompassing the monocyte (M), granulocyte (G), and lymphocyte (L) subpopulations are indicated. The means of results of such analyses for 3 mice of each genotype are shown in brackets (± SD). (B) Total pleural cavity and peritoneal cavity cells (n = 3). (C) Typical FACS analyses of CD11b⁺ peritoneal and pleural cavity cells. Percentage of positive cells for 3 mice of each genotype (± SD) is shown within each FACS distribution. FITC indicates fluorescein isothiocyanate; FSC, forward scatter; SSC, side scatter.