OxPAPC induces EGR-1 expression by an ERK1/2-dependent mechanism.

HUVECs were stimulated with 125 μg/mL OxPAPC for indicated times. Cells were collected and analyzed by Western blotting with anti–EGR-1 or anti–phospho-ERK1/2 antibody (A). (B) HUVECs were pretreated for 20 minutes with PD98059 (5 μM) and then stimulated with 125 μg/mL of OxPAPC for 15 minutes (ERK1/2) or 1 hour (EGR-1) in the presence of the inhibitor. Cells were scraped and processed for Western blotting with antibodies to phospho-ERK1/2 or EGR-1. Similar data were obtained in 3 independent experiments.