OxPAPC-induced TF activity, ERK1/2 phosphorylation, and EGR-1 expression are PKC dependent.

(A) HUVECs were preincubated for 30 minutes with indicated concentrations of the PKC inhibitor Bis I and then stimulated with 120 μg/mL OxPAPC in the presence of the inhibitor for 6 hours. TF activity was determined by clotting assay. (B) HUVECs were treated with 125 μg/mL OxPAPC in the presence of 10 μM Bis I and after 15 minutes (ERK1/2) or 1 hour (EGR-1) harvested and analyzed by Western blotting with antibodies to phospho-ERK1/2 or EGR-1. Similar results were obtained in 2 additional experiments.