OxPAPC up-regulates TF in HUVECs through Ca⁺⁺/NFAT pathway.

(A) HUVECs grown on coverslips were loaded with fura-2 as described in “Materials and methods” and then stimulated with OxPAPC or its nonoxidized precursor (PAPC, both at 125 μg/mL). The arrow indicates the time of addition of the phospholipids. The data are mean values of 8 measurements. (B) HUVECs transfected with NFAT-GFP were stimulated with OxPAPC (125 μg/mL) or Ca⁺⁺ ionophore (A23187, 4 μM). Where indicated, cells were preincubated with cyclosporin A (100 ng/mL) before stimulation with OxPAPC or Ca⁺⁺ ionophore. Intracellular localization of NFAT-GFP was determined by fluorescent microscopy 30 minutes following stimulation. Similar results were obtained in an additional independent experiment.