OxPAPC induces NFAT/DNA-binding complexes.

Nuclear extracts from HUVECs stimulated for 40 minutes with OxPAPC (125 μg/mL) or A23187/PMA (2 μM and 10⁻⁷ M, respectively) were incubated with ³²P-labeled NFAT probe of the murine IL-4 (A,B) or human TF (C) promoters and then separated by gel electrophoresis. Where indicated, cells were preincubated for 30 minutes with 500 ng/mL cyclosporin A before addition of OxPAPC or A23187/PMA. The mobility of the specific OxPAPC-induced/cyclosporin A–sensitive complex is indicated by an arrow. Supershifted band is labeled with an asterisk. The results are representative of 4 independent experiments.