cux/CDP ^ΔHD/ΔHD mice have defects in lymphoid and myeloid development.

Flow cytometry for lineage- and stage-specific cell surface markers was performed on cells isolated from wild-type/heterozygous (CT) and homozygous (ΔHD) bone marrow (BM), spleen (SP), and thymus (TH) from mice 2 to 5 weeks of age. (A) Representative FACS plots for each set of markers are shown for cells within viable gates for bone marrow and spleen and a viable lymphocyte gate for thymus. Analysis of CD25 and CD44 staining in thymus also included a lineage-negative gate. (B) Frequencies of pre-B colony-forming units in control and homozygous bone marrow were determined using stroma and IL-7 (ST) for pro-B/pre-BI cells and methylcellulose and IL-7 (ME) for pre-BII cells. Data are expressed as the percentage ofcux/CDP^ΔHD/ΔHD colonies obtained relative to control samples. (C) Comparison of the percentages of B-lineage bone marrow cells within a B220⁺ viable gate staining positive for differentiation-specific antigens c-Kit (pro-B/pre-BI), CD25 (pre-BII), and IgM (immature/mature). (D) Comparison of the numbers of erythroid (BFU-E) and myeloid colony-forming units (CFU-G/M/GM), which include granulocyte, macrophage, and mixed colonies. Studentt test P less than .05 is indicated with one asterisk and less than .005 with 2 asterisks.