Effect of rhTIMP-1 on survival of Hodgkin-derived cell line L591.

Effect of rhTIMP-1 on survival of the Hodgkin-derived cell line L591 after exposure to radiation (A,B) or H₂O₂ (B) as measured by detection of specific chromatin degradation by either nick-end labeling with dUTP and anti-BRDU and FACS analysis (A) or detection of tritium-thymidine–labeled DNA fragments in the supernatant and cytoplasm of apoptotic cells (B). (A) Shown are control conditions (i) compared with radiation conditions (ii) and rhTIMP-1 plus radiation conditions (iii). R1 indicates viable cells; and R2, apoptotic cells. Panel Aiv shows the results of an identical experiment in histogram form. (B) DNA fragmentation expressed as a percentage of values for controls in the absence of rhTIMP-1 and with use of H₂O₂ (left; 3 different experiments) or radiation (right) to induce apoptosis and cell death.