American Society of Hematology

Fig. 2.

$Fig. 2. ET-18-OCH3 induces translocation of Fas into GM1-containing lipid rafts. / Untreated Jurkat cells (Control) and Jurkat cells treated with 5 μg/mL ET-18-OCH3 for 3 hours were lysed in 1% Triton X-100 in TNEV buffer and subjected to discontinuous sucrose density gradient centrifugation. Individual fractions (20 μL) were subjected to SDS-PAGE and Western blotting. Location of GM1 and Fas was determined using CTx B subunit conjugated to horseradish peroxidase and anti-Fas polyclonal antibody, respectively, as described in “Materials and methods.” Representative blots of 3 separate experiments are shown.$

ET-18-OCH₃ induces translocation of Fas into G_M1-containing lipid rafts.

Untreated Jurkat cells (Control) and Jurkat cells treated with 5 μg/mL ET-18-OCH₃ for 3 hours were lysed in 1% Triton X-100 in TNEV buffer and subjected to discontinuous sucrose density gradient centrifugation. Individual fractions (20 μL) were subjected to SDS-PAGE and Western blotting. Location of G_M1 and Fas was determined using CTx B subunit conjugated to horseradish peroxidase and anti-Fas polyclonal antibody, respectively, as described in “Materials and methods.” Representative blots of 3 separate experiments are shown.

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