PCR amplification of factor VIII intron 1 in control and intron 1 inversion patient (UKA 29).

(A) Amplification strategy. Lines with numbers show position and length of each segment amplified. Segment 9 has been expanded to show 4 adjacent reactions, 9a to 9d. (B). PCR products from control (left) and UKA29 (right) genomic DNA. Segment 9 consistently failed to amplify in UKA29 (arrow). (C) PCR amplification of sections of segment 9 in control (left) and UKA29 DNA (right). The 1.5 kb comprising sections a to c (9ac, arrow) could not be amplified from the DNA of UKA29. However, the 1-kb overlapping segments comprising sections a + b and b + c (9ab and 9bc) were amplified as readily as the individual sections 9a to 9d.