PCR test for the FVIII

int1h-related inversion. (A) Diagram of normal (top) and inversion (bottom) DNA showing the location and orientation of relevant sequences as well as PCR primers and reactions (thin bars). (B) Amplification of int1h-1 using 9cR, 9F, andint1h-2F primers (lanes 1, 2, and 3) and int1h-2using int1h-2F, int1h-2R, and 9F primers (lanes 4, 5, and 6). Control and inversion are males; carrier is female.