American Society of Hematology

Fig. 5.

$Fig. 5. Immunophenotype of cells from the peripheral blood and spleen of FLT3-ITD mice. / Spleen (A) and blood (B) cells from mice that received bone marrow transduced with retroviruses encoding EGFP only, wild-type FLT3 and EGFP, or FLT3-ITD (mouse N78.5) and EGFP. Cells were stained with APC-conjugated anti-Gr-1 alone, APC-anti-Gr-1 and PE-conjugated Mac-1, APC-conjugated CD4 and PE-conjugated CD8 or the combination of biotinylated anti-CD19 and PE-conjugated anti-Thy-1.2 followed by APC-conjugated streptavidin. The dot plots are gated for live cells based on forward and side scatter profiles. (C) Retroviral integration. EGFP− and EGFP+ fractions of spleen cells were sorted and the purity of each population is shown using EGFP fluorescence and forward scatter. Southern analysis of these samples, where an EGFP probe demonstrates viral integration or a granzyme A probe demonstrates DNA loading, is shown on the right.$

Immunophenotype of cells from the peripheral blood and spleen of FLT3-ITD mice.

Spleen (A) and blood (B) cells from mice that received bone marrow transduced with retroviruses encoding EGFP only, wild-type FLT3 and EGFP, or FLT3-ITD (mouse N78.5) and EGFP. Cells were stained with APC-conjugated anti-Gr-1 alone, APC-anti-Gr-1 and PE-conjugated Mac-1, APC-conjugated CD4 and PE-conjugated CD8 or the combination of biotinylated anti-CD19 and PE-conjugated anti-Thy-1.2 followed by APC-conjugated streptavidin. The dot plots are gated for live cells based on forward and side scatter profiles. (C) Retroviral integration. EGFP⁻ and EGFP⁺ fractions of spleen cells were sorted and the purity of each population is shown using EGFP fluorescence and forward scatter. Southern analysis of these samples, where an EGFP probe demonstrates viral integration or a granzyme A probe demonstrates DNA loading, is shown on the right.

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