Southern analysis of proviral integration in the spleen cells of animals that received transplants of wild-type FLT3 and FLT3-ITD.

For each animal analyzed 2 digests were performed; AnXhoI digest (X) cleaves in the retroviral long terminal repeats (LTRs) releasing a fragment corresponding to the intact retrovirus and an EcoRI (E) digest cleaves within the proviral sequence and in the flanking genomic sequence allowing the clonality of the disease to be estimated. The mouse identity number for each construct is shown above the enzyme digest. The upper panel shows hybridization with a probe for EGFP. In the lower panel hybridization of the same blot with a probe for granzyme A exon I and II. The size of molecular weight marker bands is indicated on the left of each panel.