NK T cells are reduced in bi-5 mice.

(A) Flow cytometric analysis of thymocytes from a representative bi-5 mouse and littermate control that were depleted of HSA⁺ and CD8⁺ cells and analyzed in parallel. Unless otherwise stated, all data are from line 747. (B) Flow cytometric analysis of thymocytes depleted of HSA⁺ cells. This figure compares the bi-5 mice to littermate controls, CD1^−/−, and β₂M^−/− mice. The greater percentage of NK T cells in mice shown in (B) than in (A) is due in part to age-dependent differences (NK T-cell numbers increase with age) and to experimental variability. The mean percentage of HSA⁻/CD8⁻cells before bead depletion was 1.7% ± 0.8% (mean ± SD) for bi-5 and 2.8% ± 1.6% for littermate controls. Results are representative of those obtained in 4 or more independent experiments.