IEL populations in the bi-5 mice.
Flow cytometric analysis of the IELs isolated from bi-5+and littermate control mice for (A) γδ TCR versus αβ TCR and (B) CD8α versus CD8β gated on αβ−TCR+ cells. Bi-5 γδ-IELs averaged 8.3% ± 3.3% (mean ± SD), whereas littermate γδ-IELs averaged 23.5% ± 8.6% (P = .001 by unpaired Studentt test). IELs recovered from bi-5 mice were approximately 68% (range, 20%-100%) of the numbers recovered in parallel from littermate controls. The average number of IELs recovered from bi-5 mice was 4.2 × 105, which is 68% (range, 20%-100%) of the numbers recovered in parallel from littermate controls with considerable variation between experiments in cell recovery because of differences in age and the multiple processing steps. Results are representative of a minimum of 6 or more independent experiments with mice between 3 and 15 weeks of age.
