Expression of IL-2Rβ is decreased on CD4⁻CD8⁻ (DN) thymocytes of bi-5 mice.

(A) Expression of IL-2Rβ on thymocytes from bi-5 (lines 747 and 5516), β₂M^−/−, wild-type (littermate for 747 line), CD1^−/−, and CD1^−/+ mice. Thymocytes were stained with CD8-biotin, CD4-biotin, IL-2Rβ-PE, and CD44-FITC and SA-APC and were gated on the CD4⁻CD8⁻ population. Results are representative of the decrease in IL-2Rβ observed in the DN population of bi-5 thymocytes in 6 or more separate experiments. (B) Expression of IL-2Rβ on CD3⁻/CD4⁻/CD8⁻/NK1.1⁻/B220⁻thymocytes. Thymocytes were stained with CD3-APC, CD4-CyC, CD8-FITC, NK1.1-biotin, B220-biotin, SA-PharRed, and IL-2Rβ-PE. The results are representative of 2 or more separate experiments. (C) DN thymocytes from bi-5 mice and littermate controls were evaluated for the expression of a variety of cytokine receptors.