Effect of HIV gp120 on CD8⁺ cell migration.

(A) HIV-1 gp120–induced migration of CD8⁺ T cells. CD8⁺ T cells were stimulated for 3 days and examined for their ability to migrate in response to IL-16 (200 ng/mL), SDF1α (250 ng/mL), MIP1α (500 ng/mL), and gp120 (2 μg/mL) from each of 5 different HIV-1 isolates. The HIV-1 gp120s were a glycosylated version from HIV-1_SF2 (SF2 Glyc), a nonglycosylated version from HIV-1_SF2 (SF2 Non-Glyc), HIV-1_MN (MN), HIV-1_CM (CM), and HIV-1_{clade E} (clade E). Results are the percentage of cells migrating compared with medium-only controls. Asterisks indicate conditions that induced migration levels that were significantly different from those of medium-only controls (P < .05). (B) Inhibition of gp120-induced migration of CD8⁺ T cells. CD8⁺ T cells costimulated for 3 days were assessed for their ability to migrate to IL-16 (200 ng/mL) and HIV-1 gp120 (SF-2 Glyc). A mAb that inhibits gp120 interaction with CD4 (anti-CD4, Leu3a) and a mAb that inhibits gp120 interaction with CXCR4 (anti-CXCR4, 12G5) were placed in the lower chambers with gp120 in the indicated samples. The graph shows migration induced under the various conditions compared with that of medium-only controls, and asterisks indicate conditions that induced migration levels that were significantly different from those of controls (P < .05). Migration in response to IL-16 was not inhibited by 12G5 (data not shown).