Effects of STI571 and adaphostin on FDC-P1 and FDC-P1/p210^bcr/abl cells.

(A) Characterization of FDC-P1–transduced cells. Parental and p210^bcr/abl-expressing cells were sedimented at unit gravity, washed, and resuspended in medium containing or lacking WEHI-conditioned medium as a source of IL-3. After 24 hours, cells were fixed and examined for apoptotic morphologic changes. (Inset) Expression of p210^bcr/abl, Bcl-2, Bcl-x_L and histone H1 (as a loading control) in parental (lane 1) and p210^bcr/abl-transduced (lane 2) cells. (B, C) Cells were incubated for 48 hours with the indicated concentration of STI571 or adaphostin and examined for apoptotic morphologic changes. Results shown in each panel are representative of at least 3 independent experiments.