Effects of adaphostin and STI571 on CFU-G from patients with chronic-phase CML or from healthy volunteers.

(A, C) Peripheral blood mononuclear cells from 2 patients with CML (open symbols) and corresponding healthy control subjects (closed symbols) were incubated for 24 hours with the indicated concentration of adaphostin (A) or STI571 (C), washed, and plated under drug-free conditions in the presence of 50 ng/mL G-CSF. After 7 to 8 days, granulocyte colonies were counted. (B) Summary of IC₅₀values when samples from 14 patients with CML and 10 healthy control subjects were treated with adaphostin and plated in G-CSF, as illustrated in panel A. Unnumbered circles represent single samples. Numbers within circles denote multiple separate samples with the indicated IC₅₀ values.