Histogram of megakaryocyte ploidy by flow cytometry from a representative experiment.

The horizontal axis represents the intensity of fluorescence from propidium iodide expressed in a logarithmic scale, and the vertical axis represents the number of CD41⁺ cells. (A) Histogram from cultures without IL-2. (B) Histogram from cultures with IL-2 (2 ng/mL). In this experiment, the ploidy distribution in 2N, 4N, 8N, and more than 8N was 77%, 15%, 6.5%, and 1.5%, respectively, for panel A and 38%, 33%, 22%, and 7%, respectively, for panel B.