Genotyping of quiescent CD34⁺ cells resistant to STI571.

Representative RT-PCR (CML 3) demonstrating that the undivided population expressed transcripts for BCR-ABL, whether recovered from cultures with or without added growth factors. Lane 1, markers; lanes 2-5, BCR-ABL; lane 2, undivided cells from +GF culture with added STI571; lane 3, undivided cells from −GF culture with added STI571; lane 4, negative control (Neg con); lane 5, positive control (Pos con); lanes 6-9, c-ABL loading controls.