American Society of Hematology

Fig. 6.

$Fig. 6. Effect of STI571 on proliferating Ph+CD34+ cells—CML 2, example 2. / These dot-plots show representative results for a CML sample that proved to be relatively insensitive to STI571 despite more than 95% of the input cells being BCR-ABL+ by FISH. As shown in the upper left dot-plot, in the presence of added growth factors, the CD34+ cells were stimulated to proliferate up to 6 times. The addition of STI571 (upper right) reduced the number of division peaks to 4 and increased the proportion of cells in the undivided peak. In the absence of growth factors (lower left), the CD34+cells demonstrated autonomous growth with up to 3 divisions and with retention of CD34 expression. The addition of STI571 (lower right) reduced the division peaks to 2 with most cells found in the undivided fraction.$

Effect of STI571 on proliferating Ph⁺CD34⁺ cells—CML 2, example 2.

These dot-plots show representative results for a CML sample that proved to be relatively insensitive to STI571 despite more than 95% of the input cells being BCR-ABL⁺ by FISH. As shown in the upper left dot-plot, in the presence of added growth factors, the CD34⁺ cells were stimulated to proliferate up to 6 times. The addition of STI571 (upper right) reduced the number of division peaks to 4 and increased the proportion of cells in the undivided peak. In the absence of growth factors (lower left), the CD34⁺cells demonstrated autonomous growth with up to 3 divisions and with retention of CD34 expression. The addition of STI571 (lower right) reduced the division peaks to 2 with most cells found in the undivided fraction.

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