Fig. 4.
![Fig. 4. ET-1 binding in normal and sickle erythrocytes. / Specific binding of [125I]–ET-1 as a function of radiolabeled ET-1 concentration. Scatchard plot of the specific binding of iodinated ET-1 gave a Kd of 244 ± 105 nM and 336 ± 78 sites/per cell for sickle red cells. TheKd for normal red cells was 128 ± 73 nM, with a maximal binding capacity of 238 ± 53 sites/cell. No statistically significance differences were found between sickle and normal erythrocytes. The line represents the nonlinear regression of experimental points. Values are mean ± SE of 3 similar experiments.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/99/1/10.1182_blood.v99.1.357/6/h80121967004.jpeg?Expires=1735599062&Signature=t0YE~LsfTyDfe~rmxLiRlHuFwyiFkgBsU-4TMc-GJFuOaqtyIwBSuUDBFMDwl-s~BSfiYYfExySw4EDIZzkKC6gyfO7Cm2QFbU99R6~LoaJKx5N05tzYb4YYuAT1U7PP7NFB08ElLrl-x0oV3WxYdupOgHOMnhS46EGR7v3vs8t9lIwQgwjS~Vbt8tiE3-lI9~lMv6tNhy3wbfuMSmY78IOTko3yFv7tIdkaBPBe9QXLG4-gStPhPRCrbWm1GHAKzJ9vvLoa8CZVX7Fy0EASnA7jNu11uExFXt~DdjRJVjKWT~Ky3pCfpqVkE4Lh7-1SH7~LlQDC6yW5xTnyE4hfIg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
ET-1 binding in normal and sickle erythrocytes.
Specific binding of [125I]–ET-1 as a function of radiolabeled ET-1 concentration. Scatchard plot of the specific binding of iodinated ET-1 gave a Kd of 244 ± 105 nM and 336 ± 78 sites/per cell for sickle red cells. TheKd for normal red cells was 128 ± 73 nM, with a maximal binding capacity of 238 ± 53 sites/cell. No statistically significance differences were found between sickle and normal erythrocytes. The line represents the nonlinear regression of experimental points. Values are mean ± SE of 3 similar experiments.
