IRF-1 antisense ODN effect on cytokine-induced CD40 expression.

(A) Statistical summary of IRF-1 protein expression after 3 hours (calculated as percentage of cytokine-stimulated IRF-1 expression) in TNF-α (100 U/mL) plus IFN-γ (1000 U/mL)–stimulated HUVECs pretreated with an IRF-1 antisense (AS) or scrambled control (SCR) ODN, as described in “Materials and methods” (n = 3; *P < .05 versus TNF-α/IFN-γ). (B) Statistical summary of the effects of the IRF-1 antisense (AS) ODN or the corresponding missense (MS) or scrambled (SCR) control ODN on TNF-α (100 U/mL) plus IFN-γ (1000 U/mL)–stimulated CD40 mRNA expression after 9 hours (calculated as percentage of the stimulated control) in the cultured HUVECs (n = 3; *P < .05 versus TNF-α/IFN-γ). The insets show a representative Western blot and RT-PCR analysis, respectively.