Fig. 3.
![Fig. 3. Platelet-dense granule secretion is inhibited by PECAM-1 signaling. / Platelets were loaded with [3H]5-HT before stimulation with Cvx (62.5 ng/mL) or thrombin (0.1 U/mL). Where required, PECAM-1 was cross-linked an agonist was added (as described in “Materials and methods”). Secretion of [3H]5-HT into cell medium was measured using scintillation spectrometry. [3H]5-HT release is expressed as a percentage of total tissue content after subtraction of basal secretion values. Results represent mean ± SE (n = 3). Student t test was used to compare PECAM-1 cross-linked and non–cross-linked sample for statistical significance. *P < .05. PECAM-1 XL, PECAM-1 cross-linking](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/99/1/10.1182_blood.v99.1.137/6/h80121926003.jpeg?Expires=1735514931&Signature=yWgpi4Wf4L4LWfXxQ~qj~1sdggkILaoWYtnuR5Vv76damtVBNZDJtP~rgD86yH2sBXySzW9AGC-m0yzr4wty4ei7F8dAS~Tz-48W1BsQiFIB4MAz7fz0eqE8Cl37fw5WNumjpW7yA0nRBY14TAqQQUUfwABXLg0y3tBZgs8vJ-sLQvhxus08bkHunofor1C2ZSPiVX1TIaINjbFAeGz3otMK~9a8x96Uw4uYWJ4JZvafWevvte5CAk0MS8mDHf0txA~NotICZTpIGKw3nphTzcDcfnJXHf0DC2QOwQHH-yI1oXNmu7coVPa-zkqXfsZ9oGdUsN1kJLcJu0HDgHnE5A__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Platelet-dense granule secretion is inhibited by PECAM-1 signaling.
Platelets were loaded with [3H]5-HT before stimulation with Cvx (62.5 ng/mL) or thrombin (0.1 U/mL). Where required, PECAM-1 was cross-linked an agonist was added (as described in “Materials and methods”). Secretion of [3H]5-HT into cell medium was measured using scintillation spectrometry. [3H]5-HT release is expressed as a percentage of total tissue content after subtraction of basal secretion values. Results represent mean ± SE (n = 3). Student t test was used to compare PECAM-1 cross-linked and non–cross-linked sample for statistical significance. *P < .05. PECAM-1 XL, PECAM-1 cross-linking
