Analysis of hematopoietic reconstitution after transplantation of FL cells into lethally irradiated recipients.

(A) C57Bl/6 FL cells on the hemoglobin single (Hb_s) background were collected from STAT5ab^−/− and wild-type mice and counted. The cells were then injected into lethally irradiated (1100 rads) C57Bl/6 HW80 recipient mice that differ in the endogenous hemoglobin expression pattern and are hemoglobin diffuse (Hb_d). Eight to 10 weeks later the mice were bled, and the packed red blood cells were separated by electrophoresis on cellulose acetate gels. The Hb_s and Hb_d patterns of control mice that did not receive transplants are shown on the right. (B) In a second experiment, FL cells (Ly-5.2) were injected into lethally irradiated Ly-5.1 recipient mice. Shown are representative examples of mice analyzed for Ly-5.2 expression 11 weeks after transplantation. Ly-5.1 negative control and Ly-5.2 positive controls are shown in the left panels. On the right are mice that received transplants of wild-type FL (upper panel) or STAT5ab^−/−FL cells (lower panel). (C) To demonstrate a typical lineage analysis of the STAT5ab^−/− FL engraftment, gating based on forward-scatter and side-scatter profiles typical for lymphocytes (B220, CD4, and Ter119), monocytes (Mac-1), and granulocytes (Gr-1) was combined with the lineage antibodies indicated. The percentage of cells falling within the upper quadrants and the lower-right quadrant is shown.