Southern blot analysis of genomic DNA from tissues of competitive repopulation experiment no. 1 mice 6 months after transplantation.

Mice 1, 2, 4, 9, and 10 described in Figure 5 were killed 6 months after BM transplantation, and genomic DNA was prepared from the peripheral blood (PB), bone marrow (BM), and spleen. Southern blot analysis of EcoR1-digested DNA preparations was performed by using a [³²P]-labeled probe to the murine β-globin intervening sequence 2. The upper panels show a blot from the wild type competitive repopulation and the lower panels show a blot from the STAT5ab^−/− competitive repopulation. Control DNA samples were run on each Southern blot gel from mice with either the Hb_s or Hb_d and are shown on the right. Shown on the left for each gel is the DNA ladder. The larger arrow marks the correct size for the Hb_s fragment on each gel, and the small arrow marks the correct size Hb_d fragments on each gel.