CD4⁺CD25⁺ thymocytes can develop normally in μ-chain–deficient mice and NK T-cell–deficient mice.

Thymocytes from μ-chain–deficient mice, NK T-cell–deficient mice, and wild-type (WT) mice were stained with anti-CD3 FITC, anti-CD25 PE, anti-CD8 APC, and anti-CD4 PerCP. Shown are representative FACS profiles of CD4 versus CD25 on CD4⁺CD8⁻CD3^high thymocytes from 4 to 5 mice in each group. FACS profile of CD4 versus CD8 and cell number of thymocytes was indistinguishable among these mice (data not shown).