Localization and staining patterns of mitochondria in PMNs.

PMNs were incubated as described in Figure 1. Then the cells were stained with 100 nM MitoTracker Green FM and analyzed with CLSM. (A) In fresh cells the mitochondria showed a distinct tubular shape. (B) In PMNs cultured without additions the mitochondria relocalized into large unstructured aggregates. (C) G-CSF prevented the mitochondrial aggregate formation. (D) zVAD-fmk did not influence mitochondrial aggregation. Bar is 5 μm. Results are representative of at least 3 independent experiments.