Procaspase-3 cleavage in PMNs.

PMNs were incubated as described in Figure 1. Then whole cell lysates were subjected to SDS-PAGE, and Western blot was performed with an anticaspase-3 polyclonal antibody and an HRP-conjugated secondary antibody. (A) Positive control with procaspase-3 (Jurkat T cells). (B) Fresh PMNs. (C) In PMNs incubated without stimuli procaspase-3 was cleaved, and a 17-kd cleavage product appeared. (D) Addition of G-CSF considerably prevented procaspase-3 cleavage. (E) zVAD-fmk completely inhibited procaspase-3 processing. Results are representative of 3 independent experiments.