Expression levels of Bax in PMNs.

PMNs were cultured as described in Figure 1. Then the cells were fixed, permeabilized, stained with antibodies specific for Bax or with isotype control antibodies (control bars), and analyzed by FACScan. After overnight culturing, the expression of Bax had slightly increased, but this change was not significant. Treatment of the cells with either G-CSF or with zVAD-fmk had no effect on Bax expression. Data represent means ± SEM of MFI from 6 independent experiments.