![Fig. 6. Relocalization of Bax to the mitochondria in PMNs. / PMNs were cultured as described in Figure 1. Then the cells were stained with 1 μM MitoTracker Green FM, fixed, permeabilized, stained with antibody specific for Bax, and were analyzed with CLSM. (A) In fresh PMN Bax and the mitochondria were localized separately. (B) Overnight-cultured untreated cells demonstrated fusion of Bax and mitochondria into large aggregates (right panel in yellow). (C) Addition of G-CSF preserved the separate localization of Bax and mitochondria. (D) zVAD-fmk did not prevent aggregate formation and colocalization of Bax and mitochondria. (Comment: due to the fixation and permeabilization procedures, the mitochondrial staining [green] showed a more diffuse cytoplasmic pattern than the tubular structures shown in Figure 2, panels A and C). Bar is 5 μm. This figure is representative of at least 4 independent experiments.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/99/2/10.1182_blood.v99.2.672/6/h80222003006.jpeg?Expires=1735561205&Signature=hCbenxT2Y~vDjrcdk~XatH-RYtOAAcoZRATJeRmbB~ouLn8S-uJOExo2b-MjIU2BRM1pofzFfN6AMVkbVoCj5SfHJFe2qCnZ777E1IO64t-IypsVGmhdiKns6YF4OTHC8MCXI04UvOwAzENEbT1ZJKia8-EIue5cMejNtCSYkceqpEsqz96SelqvlEmDIsPAjI25v3nmBSe8Ihik6d-YLhuYzfNGCq9u~qt1DLP83eXs4e2Kw4jnD0TTrn8k7uPajAe5mJ6SVmzVlaL-9FuQnMqcph5EF4kU18qpDttvO~FFyHSdR0wP0cpgIbtXaGQNxSRp1ewsxPanzW4vSZkR3A__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Relocalization of Bax to the mitochondria in PMNs.
PMNs were cultured as described in Figure 1. Then the cells were stained with 1 μM MitoTracker Green FM, fixed, permeabilized, stained with antibody specific for Bax, and were analyzed with CLSM. (A) In fresh PMN Bax and the mitochondria were localized separately. (B) Overnight-cultured untreated cells demonstrated fusion of Bax and mitochondria into large aggregates (right panel in yellow). (C) Addition of G-CSF preserved the separate localization of Bax and mitochondria. (D) zVAD-fmk did not prevent aggregate formation and colocalization of Bax and mitochondria. (Comment: due to the fixation and permeabilization procedures, the mitochondrial staining [green] showed a more diffuse cytoplasmic pattern than the tubular structures shown in Figure 2, panels A and C). Bar is 5 μm. This figure is representative of at least 4 independent experiments.
