Activation of caspase-3 in cytoplasts.

Cytoplasts were cultured as described in Figure 7. Then the cytoplasts were fixed, permeabilized, stained with mAb specific for active caspase-3, and analyzed by CLSM. (A) Fresh cytoplasts showed faint background staining. (B,C) Overnight-cultured untreated or G-CSF–treated cytoplasts, respectively, revealed bright punctate staining representing active caspase-3. (D) Cells treated with zVAD-fmk did not demonstrate active caspase-3. Bar is 5 μm. Results are representative of at least 3 independent experiments.