Inhibition of the prosurvival effect of G-CSF in PMNs by CHX.

PMNs were cultured overnight without additions, with 5 μg/mL CHX, with 500 ng/mL G-CSF, or with the combination of CHX and G-CSF. Then the cells were stained and analyzed by FACScan as described in Figure1.(A) Untreated cell cultures overnight demonstrated a basal level of survival. (B) A low dose of CHX had no effect on basal survival. (C) G-CSF significantly increased survival (*P < .05 versus untreated cells). (D) The presence of CXH in the G-CSF culture abrogated the prosurvival effect of G-CSF (#P > .05 versus untreated cells). Values represent means ± SEM of 3 separate experiments.