Fig. 5.
Platelet PF4 mRNA levels in patient and healthy subjects.
Platelet RNA from the patient (P) and healthy subjects (H) was subject to RT-PCR in the presence of 32P-dCTPs using primers for PF4 corresponding to 48-68 nt (forward) and 313-337 nt (reverse), and for β-actin, as described in “Patient, materials, and methods.” The products were subjected to gel electrophoresis, transferred to membrane, and analyzed. Results shown are representative of 2 separate experiments.
