Fig. 1.
P-gp expression by CEM-VBL100 cells.
(A) RT-PCR and (B) Western blot analyses for P-gp expression on CCRF-CEM (1) and CEM-VBL100 (2) cells. P-gp is expressed on the CEM-VBL100 cell line, derived from CEM cells by selection in medium containing 100 ng/mL VBL, and is undetectable in the parental CEM cell line at both the mRNA (A) and protein levels (B). (C) RT-PCR and (D) Western blot analysis for ERM expression on CCRF-CEM1 and CEM-VBL1002 cells. Both cell lines expressed comparable levels of ezrin, radixin, and moesin (C,D; arrows). GAPDH (A) and actin (B) levels in the relative lysates are shown.
