Distribution of P-gp on CEM-VBL100 cells.

Control CEM-VBL100 cells presented uropoidal formations (arrow) on their surfaces (C, left panel). P-gp distribution on CEM-VBL100 cells appeared clearly polarized on these structures. The arrows point to the sites of P-gp polarization in immunocytochemistry (PAP, counterstaining with Mayer hemalum) (A), immunofluorescence (B), and immuno-SEM (C) analyses. Bar = 5 μm.