P-gp colocalization with ERM proteins.

(A) Double-staining immunofluorescence and LSCM analysis of P-gp colocalization with ezrin (left panel), radixin (central panel), and moesin (right panel). White color is the overlapping of green (P-gp) and red (ERM) signals. Bar = 1 μm. (B) A sequence of 6 optical sections obtained by the confocal analysis of a CEM-VBL100 cell stained for P-gp (green) and moesin (red) in the area of polarization squared in panel A (right panel). The most internal (upper left) to the most external (lower right) sections of the uropod double stained for moesin (red) and P-gp (green) are shown. The intermediate sections, corresponding to the juxta-membrane region, clearly showed a wide overlapping (white) of the moesin/P-gp staining.