Enhanced allostimulatory ability in adenovirus-infected monocytes.

Graded numbers of gamma-irradiated (20 Gy) monocytes or monocyte-derived DCs were added to 96-well tissue-culture plates with purified allogenic T lymphocytes (2 × 10⁵ per well). Cells were cocultured for 96 hours and pulsed with 1 μCi (.038 MBq) [³H]-TdR for an additional 18 hours; then, [³H]-TdR incorporation in harvested cells was assessed by liquid scintillation spectrometry. Results are representative of 3 separate experiments with different donors. (○) indicates uncultured monocytes; (■), monocytes cultured 72 hours in SFM/G; (▵), monocytes cultured overnight in SFM/G, infected with adenovirus-GFP, and incubated for 48 hours; (▴), monocytes that received similar treatment with heat-inactivated (HI) adenovirus-GFP; (✙), monocytes that were cultured overnight in SFM/G and treated with LPS for 48 hours.