Gene expression following infection with adenovirus vectors.

After 24 hours in SFM/G, monocytes were uninfected, were infected with an adenovirus at an MOI of 200, or were treated with 50 ng/mL LPS. (A) Monocytes harvested 48 hours after infection with adenovirus-GFP, infection with adenovirus-Luc, or treatment with LPS. Whole-cell lysates were analyzed by immunoblotting with the use of antiserum specific for RelB. The membrane was subsequently reprobed with an actin-specific antibody. (B) (C) Monocytes harvested 24 hours after infection with adenovirus-GFP or adenovirus-null. Total RNA was analyzed by RNAse protection assay. Results comparing SFM/G–, LPS–, and adenovirus-GFP–treated cells are representative of 5 separate experiments with different donors. Comparison of adenovirus-GFP and adenovirus-null was performed once.