Effects of expressed transgene, neutralizing TNF-α antibody, and NAC on adenovirus-induced monocyte differentiation.

Human monocytes were cultured for 36 hours in SFM/G and then infected with adenovirus-GFP or adenovirus-null, with or without 50 mM NAC (pretreatment for 45 minutes) or 10 μg/mL TNF-α–neutralizing mAb 5N (pretreatment for 45 minutes) or 10 μg/mL isotype-matched control antibody (isotype ct). Cells were harvested 48 hours later and analyzed by FACS. Results are representative of 5 separate experiments with different donors.